High-Level Production of Lysine in the Yeast Saccharomyces cerevisiae by Rational Design of Homocitrate Synthase-Reference-Cited by-同舟云学术

High-Level Production of Lysine in the Yeast Saccharomyces cerevisiae by Rational Design of Homocitrate Synthase

Published:2021-07-13 Issue:15 Volume:87 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Isogai Shota¹,Matsushita Tomonori¹,Imanishi Hiroyuki¹,Koonthongkaew Jirasin¹,Toyokawa Yoichi¹,Nishimura Akira¹,Yi Xiao²³,Kazlauskas Romas²³,Takagi Hiroshi¹^ORCID

Affiliation:

1. Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma, Nara, Japan

2. Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Saint Paul, Minnesota, USA

3. The BioTechnology Institute, University of Minnesota, Saint Paul, Minnesota, USA

Abstract

A traditional method for screening toxic analogue-resistant mutants has been established for the breeding of microbes that produce high levels of amino acids, including lysine. However, another efficient strategy is required to further improve their productivity.

Funder

MEXT | Japan Science and Technology Agency

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00600-21

Reference36 articles.

1. Isolation of freeze-tolerant laboratory strains of Saccharomyces cerevisiae from proline-analogue-resistant mutants

2. Lysine harvesting is an antioxidant strategy and triggers underground polyamine metabolism

3. Physiological, Biochemical, and Transcriptional Responses to Single and Combined Abiotic Stress in Stress-Tolerant and Stress-Sensitive Potato Genotypes