Replication of Measles Virus: Distinct Species of Short Nucleocapsids in Cytoplasmic Extracts of Infected Cells-Reference-Cited by-同舟云学术

Replication of Measles Virus: Distinct Species of Short Nucleocapsids in Cytoplasmic Extracts of Infected Cells

Published:1974-03 Issue:3 Volume:13 Page:721-728
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Kiley Michael P.¹,Gray Robert H.¹,Payne Francis E.¹

Affiliation:

1. Virus Laboratory, Department of Epidemiology and Department of Environmental and Industrial Health, School of Public Health, The University of Michigan, Ann Arbor, Michigan 48104

Abstract

Cytoplasmic extracts of Vero cells infected with wild-strain Edmonston measles virus were found to contain two and probably three distinct species of nucleocapsids. Species sedimenting at 200 and 110 S contained RNA which sedimented at 50 and 16 to 18 S , respectively. The third nucleocapsid species which sedimented at 170 S was not present in all experiments and was not characterized in detail. Essentially all 200 and 170 S , as well as a portion of the 110 S , nucleocapsids were membrane associated and probably present in part in cell-associated virions. Five of six plaque purified strains derived from wild-type Edmonston virus produced only 200 S nucleocapsids. One of these five plaque-purified strains subsequently produced both 200 and 110 S nucleocapsids after being passaged by using undiluted inocula. These results suggest that measles virus may produce distinct classes of defective virus containing short nucleocapsids and subgenomic viral RNA.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.13.3.721-728.1974

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