Characterization of an Endogenous RNA-Dependent DNA Polymerase Associated with Murine Intracisternal A Particles-Reference-Cited by-同舟云学术

Characterization of an Endogenous RNA-Dependent DNA Polymerase Associated with Murine Intracisternal A Particles

Published:1974-03 Issue:3 Volume:13 Page:712-720
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Yang Stringner Sue¹,Wivel Nelson A.¹

Affiliation:

1. Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

Abstract

An RNA-dependent DNA polymerase associated with intracisternal A particles has been characterized. The enzyme required Mg 2+ or Mn 2+ , dithiothreitol and the presence of all four deoxyribonucleoside triphosphates for the expression of maximal activity. Sensitivity of the endogenous RNA-dependent DNA polymerase activity to a low concentration of pancreatic ribonuclease in the presence of a high concentration of NaCl suggested that the enzyme might be utilizing the A particle endogenous RNA as template. Evidence in support of this was provided by analyses of early and late DNA products of the endogenous reaction by Cs 2 SO 4 isopycnic gradient centrifugation and hybridization of purified 60 to 70 S and 35 S RNAs of A particles with the purified DNA product.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.13.3.712-720.1974

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