The 39-kilodalton outer membrane protein of Proteus mirabilis is an OmpA protein and mitogen for murine B lymphocytes

Author:

Korn A1,Kroll H P1,Berger H P1,Kahler A1,Hessler R1,Brauburger J1,Müller K P1,Nixdorff K1

Affiliation:

1. Department of Microbiology, University of Darmstadt, Germany.

Abstract

Partial amino acid sequence analysis of a major outer membrane protein of Proteus mirabilis (39-kDa protein) indicates that it is an OmpA protein. The mitogenic activities of the 39-kDa protein for murine lymphocytes were also investigated with T lymphocytes isolated by passing spleen cells over columns of nylon wool fiber and B lymphocytes obtained by treating spleen cells with monoclonal antibodies to Thy1 plus complement. The 39-kDa protein showed little activity in stimulating T cells to proliferate but was strongly mitogenic for B cells.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference25 articles.

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2. Mitogenic activity of lipoprotein from the outer membrane of E. coli on Iymphocytes from different species;Bessler W. G.;Z. Immunitaetsforsch.,1975

3. Protein I and protein II from the outer membrane of Escherichia coli are mouse B-lymphocyte mitogens;Bessler W. G.;Z. Immunitaetsforsch.,1979

4. The nucleotide sequence coding for major outer membrane protein OmpA of Shigella dysenteriae;Braun G.;Nucleic Acids Res.,1982

5. Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12;Chen R.;Proc. Natl. Acad. Sci. USA,1980

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