Affiliation:
1. Flow Laboratories, Inc., Rockville, Maryland 20852
Abstract
The 30,000-molecular-weight internal protein, p30, was purified from seven strains of mouse type-C viruses. The individual p30's showed variation in isoelectric points and also intrastrain heterogeneity. The individual p30's could be distinguished by peptide map and quantitative complement fixation techniques with relatedness estimates of >95%. Amino terminal sequence analysis showed variability at position 4 for several p30's with complete homology otherwise through 24 residues. The intrastrain heterogeneity in p30 isoelectric points could not be explained by common contaminants, as shown by peptide mapping, and is more likely based on post-transcriptional modifications. These data provide a chemical basis for the recently described type-specific immunological properties of individual p30's.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference50 articles.
1. Independent segregation of loci for activation of biologically distinguishable RNA C-type viruses in mouse cells;Aaronson S. A.;Proc. Nat. Acad. Sci. U.S.A.,1973
2. Ambler R. P. 1972. Carboxypeptidase A and B p. 262-281. In C. H. W. Hirs and S. N. Timasheff (ed.) Methods in enzymology enzyme structure vol. 25. Academic Press Inc. New York.
3. Homology between Type C viruses of various species as determined by molecular hybridization;Benveniste R. E.;Proc. Nat. Acad. Sci. U.S.A.,1973
4. Transformation et infection chronique de cellules embryonnaires de rat par le virus du sarcome de Moloney;Bernard C. M.;C. R. Acad. Sci.,1967
5. Termination of tolerance to human gamma globulin in mice by antigen and bacterial lipopolysaccharide (Endotoxin);Chiller J. M.;J. Exp. Med.,1973
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