Affiliation:
1. Department of Biological Sciences, Louisiana State University and A & M College, Baton Rouge, Louisiana 70803
Abstract
ABSTRACT
An in vitro transposition system, developed to facilitate gene disruption in
Deinococcus radiodurans
R1, has been used to inactivate the gene designated
dr1819
in
uvrA-1
+
and
uvrA-1
backgrounds.
dr1819
encodes a protein with homology to a UV DNA damage endonuclease expressed by
Schizosaccharomyces pombe
. Interruption of
dr1819
greatly sensitizes the
uvrA-1
strain but not the
uvrA-1
+
strain to UV light, indicating that the
dr1819
gene product is a component in a DNA repair pathway that can compensate for the loss of nucleotide excision repair in this species. Clones of
dr1819
will restore UV resistance to UVS78, a
uvrA-1 uvsE
strain, indicating that
dr1819
and
uvsE
are the same locus.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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