Construction and Synchronization of dnaA Temperature-Sensitive Mutants of Streptomyces-Reference-Cited by-同舟云学术

Construction and Synchronization of dnaA Temperature-Sensitive Mutants of Streptomyces

Published:2002-02-15 Issue:4 Volume:184 Page:1214-1218
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Lee Li-Fong¹,Yeh Shun-Hua¹,Chen Carton W.¹

Affiliation:

1. Institute of Genetics, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan

Abstract

ABSTRACT Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39°C replication in the mutants ceased in about 90 min but resumed on return to 30°C. This allowed manipulations to achieve replication synchronization.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.184.4.1214-1218.2002

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