Establishment of Full-Length cDNA Clones and an Efficient Oral Infection Model for Feline Coronavirus in Cats-Reference-Cited by-同舟云学术

Establishment of Full-Length cDNA Clones and an Efficient Oral Infection Model for Feline Coronavirus in Cats

Published:2021-10-13 Issue:21 Volume:95 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Wang Gang¹²,Hu Guangli¹²,Liang Rui¹²,Shi Jiale¹²,Qiu Xiuxiu¹²,Yang Yiling¹²,Jiao Zhe¹²,Chen Yixi¹²,Shen Zhou¹²,Li Mengxia¹²,Shi Yuejun¹²,Mao Junfu³,Peng Guiqing¹²⁴^ORCID

Affiliation:

1. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China

2. Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China

3. New Ruipeng Pet Healthcare Group Co., Ltd., Beijing, China

4. College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China

Abstract

Tissue- or cell culture-adapted feline infectious peritonitis virus (FIPV) usually loses pathogenicity. To develop a highly virulent FIPV, we constructed a field isolate type I FIPV full-length clone with the spike gene replaced by the 79-1146 spike gene, corresponding to a virus named rQS-79, which induces high mortality in adult cats. rQS-79 represents the first described reverse genetics system for highly pathogenic FCoV.

Funder

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00745-21

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