Construction of Agrobacterium strains by electroporation of genomic DNA and its utility in analysis of chromosomal virulence mutations-Reference-Cited by-同舟云学术

Construction of Agrobacterium strains by electroporation of genomic DNA and its utility in analysis of chromosomal virulence mutations

Published:1994-11 Issue:11 Volume:60 Page:4192-4194
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Charles T C¹,Doty S L¹,Nester E W¹

Affiliation:

1. Department of Microbiology, University of Washington, Seattle 98195.

Abstract

We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background but differ in their types of pTi (octopine or nopaline). Both nopaline and octopine pTi-carrying chvE mutants were deficient in vir regulon induction and exhibited similar reductions in host range.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.60.11.4192-4194.1994

Reference22 articles.

1. Sugar-mediated induction ofAgrobacterium tumefaciens virulence genes: structural specificity and activities of monosaccharides;Ankenbauer R. G.;J. Bacteriol.,1990

2. Specificity ofAgrobacterium-mediated delivery of maize streak virus DNA to members of the Graminae;Boulton M. I.;Plant Mol. Biol.,1989

3. Boulton M. I. D. M. Raineri J. W. Davies and E. W. Nester. 1992. Identification of genetic factors controlling the ability of Agrobacterium to transfer DNA to maize p. 73-78. In E. W. Nester 4194 NOTES

4. Correlative association between resident plasmids and the host chromosome in a diverse Agrobacterium soil population;Bouzar H.;Appl. Environ. Microbiol.,1993

5. Sugars induce the Agrobacterium virulence genes through a periplasmic binding protein and a transmembrane signal protein;Cangelosi G. A.;Proc. Natl. Acad. Sci. USA,1990

Cited by 15 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Agrobacterial Transformation Enhancement by Improved Competent Cell Preparation and Optimized Electroporation;Life;2023-11-17

2. Inability To Catabolize Galactose Leads to Increased Ability To Compete for Nodule Occupancy in Sinorhizobium meliloti;Journal of Bacteriology;2012-07-13

3. Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE);Journal of Visualized Experiments;2012-03-16

4. Strain Engineering by Genome Mass Transfer: Efficient Chromosomal Trait Transfer Method Utilizing Donor Genomic DNA and Recipient Recombineering Hosts;Molecular Biotechnology;2009-05-20

5. Feedback regulation of an Agrobacterium catalase gene katA involved in Agrobacterium-plant interaction;Molecular Microbiology;2008-07-07