New Method for Isolating Barophiles from Intestinal Contents of Deep-Sea Fishes Retrieved from the Abyssal Zone-Reference-Cited by-同舟云学术

New Method for Isolating Barophiles from Intestinal Contents of Deep-Sea Fishes Retrieved from the Abyssal Zone

Published:1994-11 Issue:11 Volume:60 Page:4210-4212
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Nakayama Akihiko¹,Yano Yutaka¹,Yoshida Katsuhiko²

Affiliation:

1. Marine Biochemistry Division, National Research Institute of Fisheries Science, Kanazawa-ku, Yokohama, Kanagawa 236, Japan

2. Marine Productivity Division, National Research Institute of Fisheries Science, Kanazawa-ku, Yokohama, Kanagawa 236, Japan

Abstract

We devised a new method (the dorayaki method) using marine agar under in situ pressures to isolate barophilic bacteria from the intestinal contents of three deep-sea fishes (two Coryphaenoides yaquinae samples and one Ilyophis sp. sample) retrieved from depths of 4,700 to 6,100 m in the Northwest Pacific Ocean. All 10 strains isolated from one sample ( C. yaquinae ) were obligately barophilic. One of the 10 strains did not grow at atmospheric pressure and 103.4 MPa but did grow well between 20.7 and 82.7 MPa, with optimal growth at 41.4 MPa. This method is useful for isolating psychrophilic and barophilic deep-sea bacteria.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.60.11.4210-4212.1994

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