Affiliation:
1. Infectious Disease Section, Department of Medicine, Minneapolis Veterans Administration Hospital; and University of Minnesota Medical School, Minneapolis, Minnesota 55417
Abstract
The interaction of penicillin G with human serum proteins was evaluated by three different techniques: rate of dialysis, cross-linked dextran exclusion, and ultracentrifugation. The rate-of-dialysis technique demonstrated that penicillin G binding to serum was immediate but incompletely reversible. Cross-linked dextran adsorbed or trapped significant amounts of penicillin G, necessitating correction factors of more than 10%. Ultracentrifugation was found to be the most reliable method for quantitative protein-binding determinations of penicillins.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference47 articles.
1. A new approach to studying the protein-binding properties of penicillins;Acred P.;Nature (London),1963
2. Pharmacokinetics of the penicillins in man;Barza M.;Clin. Pharmacokinetics,1976
3. A high speed vacuum centrifuge suitable for the study of filterable viruses;Bauer J. H.;J. Exp. Med.,1936
4. A vacuum type air-driven centrifuge for biophysical research;Beams J. W.;Rev. Sci. Instr.,1938
5. A rapid, modified ultrafiltration method for determining serum protein binding and its application to new penicillins;Bennet J. V.;J. Lab. Clin. Med.,1965
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