Affiliation:
1. INRS—Institut Armand-Frappier, 531 Boul. des Prairies, Laval, Québec, Canada H7V 1B7
Abstract
ABSTRACT
Streptomyces lividans
is a Gram-positive bacterium known for its remarkable secretion efficiency and low extracellular protease activity. In the present work, we adapted the highly productive T7 expression system to
S. lividans
. A codon-optimized T7 RNA polymerase gene was chromosomally integrated, and a bifunctional T7 expression vector was constructed.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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