Identification of type D Pasteurella multocida by counterimmunoelectrophoresis

Author:

Chengappa M M,Carter G R,Bailie W E

Abstract

A counterimmunoelectrophoresis (CIE) test was applied to serotype 35 isolates of type D Pasteurella multocida recovered from 32 cases of atrophic rhinitis (in swine) and 3 cases of snuffles (in rabbits). The CIE test was compared with the indirect hemagglutination (IHA) and acriflavine (AF) tests. Results of the CIE test correlated 100% with those of the IHA test whereas results of the AF test correlated 91.43% with those of the IHA test. The CIE test was rapid and simpler to perform compared with the IHA test and more sensitive than the AF test. Cross-reactions were not encountered with capsular antigens of P. multocida types A, B, and E in the CIE test. The CIE test was not found to be suitable for typing type A P. multocida strains.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference13 articles.

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4. A new serologic type of Pasteurella multocida;Carter G. R.;Vet. Rec.,1961

5. Carter G. R. 1984. Serotyping of Pasteurella multocida p. 247-258. In T. Bergan (ed.) Methods in microbiology vol. 16. Academic Press Inc. London.

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