Affiliation:
1. Department of Biological Sciences, University of Wisconsin—Milwaukee, Milwaukee, Wisconsin
Abstract
ABSTRACT
A modified
mariner
transposon, mini
Himar RB1
, was generated to mutagenize cells of the metal-reducing bacterium
Shewanella oneidensis
. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon insertions in the cytochrome
c
maturation genes
ccmC
and
ccmF1
, respectively, were analyzed. Both mutants were deficient in anaerobic respiration and cytochrome
c
production.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology