Differentiation of Phytophthora infestans Sporangia from Other Airborne Biological Particles by Flow Cytometry-Reference-Cited by-同舟云学术

Differentiation of Phytophthora infestans Sporangia from Other Airborne Biological Particles by Flow Cytometry

Published:2002-01 Issue:1 Volume:68 Page:37-45
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Day Jennifer P.¹,Kell Douglas B.¹,Griffith Gareth W.¹

Affiliation:

1. Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, Wales SY23 3DA, United Kingdom

Abstract

ABSTRACT The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or Botrytis spp., rust urediniospores, and pollen of grasses and plantain. Differentiation between P. infestans sporangia and powdery mildew conidia was not possible by these two methods but, when combined with analytical rules evolved by genetic programming methods, could be achieved after staining with the fluorescent brightener Calcofluor white M2R. The potential application of these techniques to the prediction of late-blight epiphytotics in the field is discussed.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.1.37-45.2002

Reference45 articles.

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