Competitive PCR for Quantitation of a Cytophaga-Flexibacter-Bacteroides Phylum Bacterium Associated with the Tuber borchii Vittad. Mycelium-Reference-Cited by-同舟云学术

Competitive PCR for Quantitation of a Cytophaga-Flexibacter-Bacteroides Phylum Bacterium Associated with the Tuber borchii Vittad. Mycelium

Published:2002-12 Issue:12 Volume:68 Page:6421-6424
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Barbieri Elena¹,Riccioni Giulia¹,Pisano Anna¹,Sisti Davide²,Zeppa Sabrina¹,Agostini Deborah¹,Stocchi Vilberto¹

Affiliation:

1. “Giorgio Fornaini” Institute of Biochemistry

2. Institute of Botany, University of Urbino, 61029 Urbino, Pesaro-Urbino, Italy

Abstract

ABSTRACT An uncultured bacterium associated with the ectomycorrhizal fungus Tuber borchii Vittad. was identified as a novel member of the Cytophaga-Flexibacter-Bacteroides group. Utilizing a quantitative PCR targeting the 16S rRNA gene, we relatively quantified this bacterium in the host. The estimated number of bacteria was found to be approximately 10 6 cells per 30-day-old T. borchii mycelium culture. This represents the first molecular attempt to enumerate an uncultured bacterium associated with a mycorrhizal fungus.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.12.6421-6424.2002

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