Affiliation:
1. Laboratory of Microbiology and Biotechnology of Foods
2. Laboratory of Dairy Research, Department of Food Science and Technology, Agricultural University of Athens, Athens 118 55, Greece
Abstract
ABSTRACT
The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (
Sparus
aurata
) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106
Pseudomonas
strains isolated from
S.
aurata
stored under different temperatures (at 0, 10, and 20°C) and packaging conditions (air and a modified atmosphere of 40% CO
2
-30% N
2
-30% O
2
).
Pseudomonas lundensis
was the predominant species, followed by
Pseudomonas fluorescens
, while
Pseudomonas fragi
and
Pseudomonas putida
were detected less frequently. Fluorescent
Pseudomonas
strains dominated under air conditions, while proteolytic and less lipolytic strains dominated under modified-atmosphere packaging. Different storage conditions appear to govern the selection of pseudomonads in gilt-head sea bream fish.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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