Affiliation:
1. Laboratory of Microbiology and Biotechnology of Foods
2. Laboratory of Dairy Research, Department of Food Science and Technology, Agricultural University of Athens, Athens 118 55, Greece
Abstract
ABSTRACT
The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (
Sparus
aurata
) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106
Pseudomonas
strains isolated from
S.
aurata
stored under different temperatures (at 0, 10, and 20°C) and packaging conditions (air and a modified atmosphere of 40% CO
2
-30% N
2
-30% O
2
).
Pseudomonas lundensis
was the predominant species, followed by
Pseudomonas fluorescens
, while
Pseudomonas fragi
and
Pseudomonas putida
were detected less frequently. Fluorescent
Pseudomonas
strains dominated under air conditions, while proteolytic and less lipolytic strains dominated under modified-atmosphere packaging. Different storage conditions appear to govern the selection of pseudomonads in gilt-head sea bream fish.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference67 articles.
1. Anzai, Y., Y. Kudo, and H. Oyaizu. 1997. The phylogeny of the genera Chryseomonas,Flavimonas and Pseudomonas supports synonymy of those three genera. Int. J. Syst. Bacteriol.47:249–251.
2. Ayres, J. C. 1960. The relationship of organisms of the genus Pseudomonas to the spoilage of meat, poultry and eggs. J. Appl. Bacteriol.23:471–486.
3. Banks, J. G., and R. G. Board. 1983. The classification of pseudomonads and other obligately aerobic Gram-negative bacteria from British pork sausage and ingredients. Syst. Appl. Microbiol.4:424–438.
4. Baranyi, J., T. A. Roberts, and P. McClure. 1993. A non-autonomous differential equation to model bacterial growth. Food Microbiol.10:43–59.
5. Barrett, E., R. E. Solanes, J. Tang, and N. J. Palleroni. 1986. Pseudomonasfluorescens biovar V: its resolution into distinct component groups and the relationship of these groups to other P.fluorescens biovars, to P. putida and to psychrotrophic pseudomonads associated with food spoilage. J. Gen. Microbiol.132:2709–2721.
Cited by
115 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献