Affiliation:
1. Department of Civil and Environmental Engineering, University of Wisconsin—Madison, Madison, Wisconsin 53706-1691
Abstract
ABSTRACT
Nitrification in drinking water distribution systems is a common operational problem for many utilities that use chloramines for secondary disinfection. The diversity of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the distribution systems of a pilot-scale chloraminated drinking water treatment system was characterized using terminal restriction fragment length polymorphism (T-RFLP) analysis and 16S rRNA gene (ribosomal DNA [rDNA]) cloning and sequencing. For ammonia oxidizers, 16S rDNA-targeted T-RFLP indicated the presence of
Nitrosomonas
in each of the distribution systems, with a considerably smaller peak attributable to
Nitrosospira
-like AOB. Sequences of AOB amplification products aligned within the
Nitrosomonas oligotropha
cluster and were closely related to
N. oligotropha
and
Nitrosomonas ureae
. The nitrite-oxidizing communities were comprised primarily of
Nitrospira
, although
Nitrobacter
was detected in some samples. These results suggest a possible selection of AOB related to
N. oligotropha
and
N. ureae
in chloraminated systems and demonstrate the presence of NOB, indicating a biological mechanism for nitrite loss that contributes to a reduction in nitrite-associated chloramine decay.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
196 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献