Affiliation:
1. Division of Bacteriology and Parasitology, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, Louisiana
Abstract
ABSTRACT
bmp
gene family 36 of
Borrelia burgdorferi
, the agent of Lyme disease, comprises four paralogs:
bmpA
,
bmpB
,
bmpC
, and
bmpD
. The
bmpA
and
bmpB
genes constitute an operon. All four genes have been found to be transcribed in cultured spirochetes. Expression from the
bmpAB
operon results in three distinct transcripts of 1.1, 1.6, and 2.4 kb, and the relative expression of
bmpA
mRNA is three- to fourfold greater than that of
bmpB
mRNA. However, thus far only expression of the BmpA protein has been demonstrated. Therefore, in this study we characterized the origins of the three transcripts and compared the relative expression of the BmpA and BmpB proteins. Northern blotting revealed that the three distinct transcripts originated from a single promoter located upstream of
bmpA
but terminated either 3′ to the
bmpA
(1.1-kb RNA) or
bmpB
(2.4-kb RNA) gene or, most unusually, within the
bmpB
gene (1.6-kb RNA). Termination within the
bmpB
gene was associated with a functional Rho-independent transcription terminator. At the protein level, we also observed a 4.3-fold greater abundance of BmpA compared to that of BmpB. These studies identify a transcription termination mechanism in
B. burgdorferi
resulting in the disparate expression of the two genes of the
bmpAB
operon.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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