Utility of Reverse Transcriptase PCR for Rapid Diagnosis of Influenza A Virus Infection and Detection of Amantadine-Resistant Influenza A Virus Isolates

Author:

Pachucki Constance T.1,Khurshid M. Aijaz1,Nawrocki John1

Affiliation:

1. Department of Infectious Disease and Department of Laboratory Medicine and Pathology, Edward Hines Jr. Veterans Administration Hospital, Hines, Illinois

Abstract

ABSTRACT A reverse transcriptase PCR was developed to detect 50 or 5,000 RNA copies of influenza A virus per ml in throat swab specimens. The assay was more sensitive than the Directigen Flu A test. The technique was also used to detect amantadine-resistant isolates.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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