Affiliation:
1. Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain
Abstract
ABSTRACT
Colletotrichum
species have caused human infections in recent years. Because of the difficulties in recognizing them in vitro, we have designed a quick and unambiguous molecular test, based on the amplification of a specific fragment of the internal transcribed spacer 1 region, to distinguish any
Colletotrichum
isolate from other fungi, including the common pathogenic species. Analysis of the sequences of the ribosomal DNA (rDNA) fragment showed sufficient variability to clearly separate the five species of
Colletotrichum
that are of clinical interest, i.e.,
Colletotrichum coccodes
,
C. crassipes
,
C. dematium
,
C. gloeosporioides
, and
C. graminicola.
Sequencing of the D1-D2 region of the large-subunit rDNA gene also supported these results. Additionally, we reviewed the most suitable morphological characteristics for the in vitro identification of these increasingly important opportunistic fungi.
Publisher
American Society for Microbiology
Cited by
103 articles.
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