Use of a Recombinant Envelope Protein Subunit Antigen for Specific Serological Diagnosis of West Nile Virus Infection

Author:

Beasley David W. C.1,Holbrook Michael R.1,Travassos da Rosa Amelia P. A.1,Coffey Lark1,Carrara Anne-Sophie1,Phillippi-Falkenstein Kathrine2,Bohm Rudolf P.2,Ratterree Marion S.2,Lillibridge Kristy M.3,Ludwig George V.4,Estrada-Franco Jose1,Weaver Scott C.1,Tesh Robert B.1,Shope Robert E.1,Barrett Alan D. T.1

Affiliation:

1. Center for Biodefense and Emerging Infectious Diseases, Sealy Center for Vaccine Development, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0609

2. Tulane National Primate Research Center, Tulane University, Covington, Louisiana 70433

3. UT Houston School of Public Health, Houston, Texas 77030

4. Diagnostic Systems and Virology Divisions, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011

Abstract

ABSTRACT Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference25 articles.

1. Anonymous. 2003. West Nile virus activity—United States, November 20-25, 2003. Morb. Mortal. Wkly. Rep.52:1160.

2. Identification of Neutralizing Epitopes within Structural Domain III of the West Nile Virus Envelope Protein

3. Beaty B. J. C. H. Calisher and R. E. Shope. 1995. Arboviruses p. 189-212. In E. H. Lennette D. A. Lennette and E. T. Lennette (ed.) Diagnostic procedures for viral Rickettsial and chlamydial infections 7th ed. American Public Health Association Washington D.C.

4. Biophysical Characterization and Vector-Specific Antagonist Activity of Domain III of the Tick-Borne Flavivirus Envelope Protein

5. Centers for Disease Control and Prevention. 2003. Epidemic/epizootic West Nile virus in the United States: guidelines for surveillance prevention and control 3rd revision. U.S. Department of Health and Human Services Public Health Service Centers for Disease Control and Prevention National Center for Infectious Diseases Division of Vector-Borne Infectious Diseases Fort Collins Colo..

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