Evaluation of PNA-FISH Yeast Traffic Light for Rapid Identification of Yeast Directly from Positive Blood Cultures and Assessment of Clinical Impact

Abstract

ABSTRACT The PNA-FISH Yeast Traffic Light assay was performed on 54 clinical isolates of yeasts inoculated into blood culture bottles. The assay showed high sensitivity ( Candida albicans/C. parapsilosis , 100%; C. glabrata/C. krusei , 92.3%; C. tropicalis , 100%) and specificity ( C. albicans/C. parapsilosis , 100%; C. glabrata/C. krusei , 94.8%; C. tropicalis , 100%). Case note review estimated a change in therapy in 29% of cases had the PNA-FISH result been available to the clinician.