Improved glycine-extracted complement-fixing antigen for human cytomegalovirus

Author:

Kettering J D,Schmidt N J,Lennette E H

Abstract

High-titered, sensitive, and stable complement-fixing antigens for human cytomegalovirus were consistently produced from human diploid fibroblast cells infected at a high multiplicity of virus, harvested after 7 days of incubation, and sonically treated immediately in 0.1 M glycine buffer, pH 9.5.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference6 articles.

1. Complementfixing antibody in human sera reactive with viral and soluble antigens of cytomegalovirus;Cremer N. E.;J. Clin. Microbiol.,1975

2. Hayflick L. 1973. Fetal human diploid cells p. 43-45. In P. F. Kruse Jr. and M. K. Patterson Jr. (ed.) NOTES 649

3. Complement-fixing antigen of human cytomegalovirus;Kim K. S.;J. Infect. Dis.,1977

4. A study of complement fixing, immunofluorescent, and neutralizing antibodies in human cytomegalovirus infections;Krech U.;Z. Immunitaesforsch. Allerg. Klin. Immunol.,1971

5. Lennette E. H. 1969. General principles underlying laboratory diagnosis of viral and rickettsial infections p. 52-58. In E. H. Lennette and N. J. Schmidt (ed.) Diagnostic procedures for viral and rickettsial infections 4th ed. American Public Health Association Inc. New York.

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