Integration Site for Streptomyces Phage φBT1 and Development of Site-Specific Integrating Vectors-Reference-Cited by-同舟云学术

Integration Site for Streptomyces Phage φBT1 and Development of Site-Specific Integrating Vectors

Published:2003-09 Issue:17 Volume:185 Page:5320-5323
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Gregory Matthew A.¹,Till Rob¹,Smith Margaret C. M.¹

Affiliation:

1. Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom

Abstract

ABSTRACT Despite extensive similarities between the genomes of the Streptomyces temperate phages φC31 and φBT1, the attP-int loci are poorly conserved. Here we demonstrate that φBT1 integrates into a different attachment site than φC31. φBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on φBT1 integrase were shown to have a broad host range and are fully compatible with those based on the φC31 attP-int locus.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.185.17.5320-5323.2003

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