An Integrated Structural and Computational Study of the Thermostability of Two Thioredoxin Mutants from Alicyclobacillus acidocaldarius-Reference-Cited by-同舟云学术

An Integrated Structural and Computational Study of the Thermostability of Two Thioredoxin Mutants from Alicyclobacillus acidocaldarius

Published:2003-07-15 Issue:14 Volume:185 Page:4285-4289
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Bartolucci Simonetta¹,De Simone Giuseppina²,Galdiero Stefania¹,Improta Roberto²,Menchise Valeria²,Pedone Carlo²,Pedone Emilia²,Saviano Michele²

Affiliation:

1. Dipartimento di Chimica Biologica, University of Naples “Federico II,”

2. Istituto di Biostrutture e Bioimmagini-CNR, 80134 Naples, Italy

Abstract

ABSTRACT We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.185.14.4285-4289.2003

Reference48 articles.

1. Adamo, C., and V. Barone. 1999. Toward reliable density functionals without adjustable parameters: the PBE0 model. J. Chem. Phys.110:6158-6170.

2. Amovilli, C., V. Barone, R. Cammi, E. Cances, M. Cossi, B. Mennucci, C. S. Pomelli, and J. Tomasi. 1998. Recent advances in the description of solvent effects with the polarizable continuum model. Adv. Quantum Chem.32:227-262.

3. Barone, V., and M. Cossi. 1998. Quantum calculation of molecular energies and energy gradients in solution by a conductor solvent model. J. Phys. Chem. A102:1995-2001.

4. Barone, V. M. Cossi, and J. Tomasi. 1997. A new definition of cavities for the computation of solvation free energies by the polarizable continuum model. J. Chem. Phys.107:3210-3221.

5. Bartolucci, S., A. Guagliardi, E. Pedone, D. de Pascale, R. Cannio, L. Camardella, M. Rossi, G. Nicastro, C. de Chiara, P. Facci, G. Mascetti, and C. Nicolini. 1997. Thioredoxin from Bacillus acidocaldarius: characterization, high-level expression in Escherichia coli and molecular modelling. Biochem. J.328:277-285.

Cited by 8 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Corrigendum for Pedone E, Limauro D, and Bartolucci S. The Machinery for Oxidative Protein Folding in Thermophiles.Antioxid Redox Signal10:157–169, 2008;Antioxidants & Redox Signaling;2010-01

2. On the origin of life in the Zinc world. 2. Validation of the hypothesis on the photosynthesizing zinc sulfide edifices as cradles of life on Earth;Biology Direct;2009

3. The Machinery for Oxidative Protein Folding in Thermophiles;Antioxidants & Redox Signaling;2008-01

4. Protein thermal stability: The role of protein structure and aqueous environment;Archives of Biochemistry and Biophysics;2007-10

5. Crystal structure of the yeast cytoplasmic thioredoxin Trx2;Proteins: Structure, Function, and Bioinformatics;2006-10-16