Experimental Determination and System Level Analysis of Essential Genes in Escherichia coli MG1655-Reference-Cited by-同舟云学术

Experimental Determination and System Level Analysis of Essential Genes in Escherichia coli MG1655

Published:2003-10 Issue:19 Volume:185 Page:5673-5684
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Gerdes S. Y.¹,Scholle M. D.¹,Campbell J. W.¹,Balázsi G.²,Ravasz E.³,Daugherty M. D.¹,Somera A. L.²,Kyrpides N. C.¹,Anderson I.¹,Gelfand M. S.¹,Bhattacharya A.¹,Kapatral V.¹,D'Souza M.¹,Baev M. V.¹,Grechkin Y.¹,Mseeh F.¹,Fonstein M. Y.¹,Overbeek R.¹,Barabási A.-L.³,Oltvai Z. N.²,Osterman A. L.¹

Affiliation:

1. Integrated Genomics, Inc., Chicago, Illinois 60612

2. Department of Pathology, Northwestern University, Chicago, Illinois 60611

3. Department of Physics, University of Notre Dame, Notre Dame, Indiana 46556

Abstract

ABSTRACT Defining the gene products that play an essential role in an organism's functional repertoire is vital to understanding the system level organization of living cells. We used a genetic footprinting technique for a genome-wide assessment of genes required for robust aerobic growth of Escherichia coli in rich media. We identified 620 genes as essential and 3,126 genes as dispensable for growth under these conditions. Functional context analysis of these data allows individual functional assignments to be refined. Evolutionary context analysis demonstrates a significant tendency of essential E. coli genes to be preserved throughout the bacterial kingdom. Projection of these data over metabolic subsystems reveals topologic modules with essential and evolutionarily preserved enzymes with reduced capacity for error tolerance.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.185.19.5673-5684.2003

Reference39 articles.

1. Akerley, B. J., E. J. Rubin, V. L. Novick, K. Amaya, N. Judson, and J. J. Mekalanos. 2002. A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc. Natl. Acad. Sci. USA99:966-971.

2. Anderson, R. P., and J. R. Roth. 1978. Tandem chromosomal duplications in Salmonella typhimurium: fusion of histidine genes to novel promoters. J. Mol. Biol.119:147-166.

3. Badarinarayana, V., P. W. Estep III, J. Shendure, J. Edwards, S. Tavazoie, F. Lam, and G. M. Church. 2001. Selection analyses of insertional mutants using subgenic-resolution arrays. Nat. Biotechnol.19:1060-1065.

4. The Complete Genome Sequence of Escherichia coli K-12

5. Csete, M. E., and J. C. Doyle. 2002. Reverse engineering of biological complexity. Science295:1664-1669.

Cited by 639 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Site-directed mutagenesis of bifunctional riboflavin kinase/FMN adenylyltransferase via CRISPR/Cas9 to enhance riboflavin production;Synthetic and Systems Biotechnology;2024-09

2. Deciphering the pH-dependent oligomerization of aspartate semialdehyde dehydrogenase from Wolbachia endosymbiont of Brugia malayi: An in vitro and in silico approaches;International Journal of Biological Macromolecules;2024-09

3. Noise robustness and metabolic load determine the principles of central dogma regulation;Science Advances;2024-08-23

4. Systematic development of a highly efficient cell factory for 5-aminolevulinic acid production;Trends in Biotechnology;2024-08

5. Differentially used codons among essential genes in bacteria identified by machine learning-based analysis;Molecular Genetics and Genomics;2024-07-27