Biochemical Function of msl5 ( pks8 plus pks17 ) in Mycobacterium tuberculosis H37Rv: Biosynthesis of Monomethyl Branched Unsaturated Fatty Acids-Reference-Cited by-同舟云学术

Biochemical Function of msl5 ( pks8 plus pks17 ) in Mycobacterium tuberculosis H37Rv: Biosynthesis of Monomethyl Branched Unsaturated Fatty Acids

Published:2003-08 Issue:15 Volume:185 Page:4620-4625
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Dubey Vinod S.¹,Sirakova Tatiana D.¹,Cynamon Michael H.²,Kolattukudy Pappachan E.¹

Affiliation:

1. Biomolecular Science Center and Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, Florida 32816

2. Department of Medicine, Veterans Affairs Medical Center, Syracuse, New York 13210

Abstract

ABSTRACT We show that the disruption of one of the mycocerosic acid synthase ( mas )-like genes, msl5 ( pks8 plus pks17 ) in Mycobacterium tuberculosis H37Rv generates a mutant incapable of producing monomethyl branched unsaturated C 16 to C 20 fatty acids that are minor constituents of acyltrehaloses and sulfolipids. The msl5 mutation did not cause any significant change in the acyl lipid composition and also did not affect growth in culture, in mouse alveolar macrophage cell line MH-S, or in the murine lung.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.185.15.4620-4625.2003

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