Evaluation of the Modified Broth-Disk Method for Determining Antibiotic Susceptibilities of Anaerobic Bacteria

Author:

Blazevic Donna J.1

Affiliation:

1. Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota 55455

Abstract

A total of 110 strains of anaerobic bacteria including 9 different genera was tested for antibiotic susceptibility by the modified broth-disk method of Wilkins and Thiel (1973), using prereduced media and the VPI anaerobic culture system. Results were compared with those obtained with an agar dilution technique with incubation in a GasPak jar. There was greater than 95% agreement between the two methods with ampicillin, cephalothin, chloramphenicol, and clindamycin, and 90% or greater agreement with erythromycin, penicillin, and tetracycline.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference9 articles.

1. Antibiotic susceptibility testing by a single disc method;Bauer A. W.;Am. J. Clin. Pathol.,1966

2. Quality control testing with disk antibiotic susceptibility test of Bauer-Kirby-Sherris-Turck;Blazevic D. J.;Am. J. Clin. Pathol.,1972

3. Holdeman L. V. and W. E. C. Moore (ed.). 1973. Anaerobe laboratory manual. Virginia Polytechnic Institute Anaerobe Laboratory Blackburg Va.

4. The effect of carbon dioxide on the sensitivity of Bacteroides fragilis to certain antibiotics in vitro;Ingham H. R.;J. Clin. Pathol.,1970

5. Matsen J. M. and A. L. Barry. 1974. Susceptibility testing: diffusion test procedures p. 418-427. In E. H. Lennette E. H. Spaulding and J. P. Truant (ed.) Manual of clinical microbiology 2nd ed. American Society for Microbiology Washington D.C.

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