Affiliation:
1. Department of Biology, University of California, San Diego, La Jolla, California 92037
Abstract
In previous work, at least 10 discrete cistrons involved in the synthesis of flagella in
Escherichia coli
were described. Six cistrons were located between
his
and
uvrC
on the genetic map. These were referred to as
hag, flaA, flaB, flaC, flaD
, and
flaE
. Four cistrons referred to as
mot, flaG, flaH
, and
flaI
were located between
uvrC
and
aroD
. In order to determine whether these genes are organized into transcriptional units, a series of Mu phage-induced flagellar mutants was studied. The mutant strains behaved as if they were carrying strong polar mutations. Of 228 independent Mu-induced mutants, 114 with mutations in the
his-aroD
region of the genetic map were tested by preparing partial diploid strains with episomes carrying a variety of previously defined mutations. The pattern of complementation that emerged indicated that cistrons
flaB, flaC
, and
flaE
form a transcriptional unit. Cistron
flaO
, defined in the course of this study, is also a member of this transcriptional unit. The order of transcription is
B-C-O-E. flaA
was found to be complex, and it included four cistrons,
flaA, flaP, flaQ
, and
flaR
, with the transcriptional order
A-P-Q-R
. Cistrons
flaG
and
flaH
are cotranscribed with the transcriptional order
G-H
. The remaining genes,
flaD, flaI, hag
, and
mot
do not belong to multicistronic transcriptional units. Complementation analysis suggested that the
cheC
locus is the same as cistron
flaA
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
59 articles.
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