Growth in High Osmotic Medium of an Unsaturated Fatty Acid Auxotroph of Escherichia coli K-12

Author:

Broekman J. H. F. F.1,Steenbakkers Johan F.1

Affiliation:

1. Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands

Abstract

A temperature-sensitive mutant of Escherichia coli K-12, which is impaired in the biosynthesis of the unsaturated fatty acids at 42 C, continues to grow and remains viable in a medium with a high osmotic pressure. Under these growth conditions, 50% of the fatty acids of the parental strain and 80% of the fatty acids of the mutant strain are saturated.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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