Age-Dependent Ribosomal DNA Variations in Mice-Reference-Cited by-同舟云学术

Age-Dependent Ribosomal DNA Variations in Mice

Published:2020-10-26 Issue:22 Volume:40 Page:
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Watada Eriko¹²,Li Sihan³,Hori Yutaro¹,Fujiki Katsunori¹,Shirahige Katsuhiko¹⁴,Inada Toshifumi³,Kobayashi Takehiko¹²⁴^ORCID

Affiliation:

1. Institute for Quantitative Biosciences, University of Tokyo, Tokyo, Japan

2. Department of Biological Sciences, University of Tokyo, Tokyo, Japan

3. Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan

4. Collaborative Research Institute for Innovative Microbiology, University of Tokyo, Tokyo, Japan

Abstract

The rRNA gene, which consists of tandem repetitive arrays (ribosomal DNA [rDNA] repeat), is one of the most unstable regions in the genome. The rDNA repeat in the budding yeast Saccharomyces cerevisiae is known to become unstable as the cell ages. However, it is unclear how the rDNA repeat changes in aging mammalian cells. Using quantitative single-cell analyses, we identified age-dependent alterations in rDNA copy number and levels of methylation in mice. The degree of methylation and copy number of rDNA from bone marrow cells of 2-year-old mice were increased by comparison to levels in 4-week-old mice in two mouse strains, BALB/cA and C57BL/6.

Funder

Japan Agency for Medical Research and Development

MEXT | Japan Society for the Promotion of Science

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.00368-20

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