Impact of CO 2 /HCO 3 – Availability on Anaplerotic Flux in Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Strains-Reference-Cited by-同舟云学术

Impact of CO 2 /HCO 3 – Availability on Anaplerotic Flux in Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Strains

Published:2019-10-15 Issue:20 Volume:201 Page:
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Krüger Aileen¹,Wiechert Johanna¹,Gätgens Cornelia¹,Polen Tino¹,Mahr Regina²,Frunzke Julia¹^ORCID

Affiliation:

1. Institut für Bio- und Geowissenschaften, IBG-1: Biotechnology, Forschungszentrum Jülich, Jülich, Germany

2. SenseUp GmbH, Forschungszentrum Jülich, Jülich, Germany

Abstract

CO 2 is a ubiquitous product of cellular metabolism and an essential substrate for carboxylation reactions. The pyruvate dehydrogenase complex (PDHC) catalyzes a central metabolic reaction contributing to the intracellular CO 2 /HCO 3 – pool in many organisms. In this study, we used a PDHC-deficient strain of Corynebacterium glutamicum , which additionally lacked pyruvate carboxylase (Δ aceE Δ pyc ). This strain featured a >15-h lag phase during growth on glucose-acetate mixtures. We used this strain to systematically assess the impact of alterations in the intracellular CO 2 /HCO 3 – pool on growth in glucose-acetate medium. Remarkably, all measures enhancing CO 2 /HCO 3 – levels successfully restored growth. These results emphasize the strong impact of the intracellular CO 2 /HCO 3 – pool on metabolic flux, especially in strains exhibiting low endogenous CO 2 production rates.

Funder

Helmholtz Association

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00387-19

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