Transduction of a Plasmid Carrying the Cohesive End Region from Lactococcus lactis Bacteriophage ΦLC3

Author:

Birkeland Nils-Kåre1,Holo Helge1

Affiliation:

1. Laboratory of Microbial Gene Technology, The Norwegian Research Council, P.O. Box 5051, N-1432 Ås, Norway

Abstract

Plasmids carrying the cohesive end region from temperate lactococcal bacteriophage ΦLC3 could be packaged in vivo by ΦLC3 and transduced into its host strain, Lactococcus lactis subsp. cremoris NCDO 1201. The transduction frequencies were between 10 -4 and 10 -3 transducing particles per PFU, depending on the size of the phage DNA insert. This transduction system is limited to only certain lactococcal strains. The ΦLC3 cohesive site region ( cos ) appears to play an important role in plasmid transduction.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference31 articles.

1. Birkeland N. K. Unpublished data.

2. Taxonomic differentiation of bacteriophages of Lactococcus lactis by electron microscopy, DNA-DNA hybridization, and protein profiles;Braun V.;J. Gen. Microbiol.,1989

3. Two plasmid determined restriction and modification systems in Streptococcus lactis;Chopin A.;Plasmid,1984

4. Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage X heads;Collins J.;Proc. Natl. Acad. Sci. USA,1978

5. Coveney J. A. G. F. Fitzgerald and C. Daly. 1987. Detailed characterization and comparison of four lactic streptococcal 1968 NOTES

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