Infection of primary cultures of human neural cells by human coronaviruses 229E and OC43

Author:

Bonavia A1,Arbour N1,Yong V W1,Talbot P J1

Affiliation:

1. Laboratory of Neuroimmunovirology, University of Quebec, Laval, Canada.

Abstract

We evaluated the ability of human coronaviruses to infect primary cultures of human neural cells. Double immunofluorescence with antibodies to virus and cell markers showed infection of fetal astrocytes and of adult microglia and astrocytes by strain OC43. RNA amplification revealed infection of fetal astrocytes, adult microglia, and a mixed culture of adult oligodendrocytes and astrocytes by strain 229E. Infectious virus was released only from fetal astrocytes, with higher titers for OC43. Human coronaviruses have the capacity to infect some cells of the central nervous system, although infection of adult cells appears abortive.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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