Application of Long Sequence Reads To Improve Genomes for Clostridium thermocellum AD2, Clostridium thermocellum LQRI, and Pelosinus fermentans R7

Author:

Utturkar Sagar M.1,Bayer Edward A.2,Borovok Ilya3,Lamed Raphael3,Hurt Richard A.4,Land Miriam L.4,Klingeman Dawn M.45,Elias Dwayne4ORCID,Zhou Jizhong6,Huntemann Marcel7,Clum Alicia7,Pillay Manoj7,Palaniappan Krishnaveni7,Varghese Neha7,Mikhailova Natalia7,Stamatis Dimitrios7,Reddy T. B. K.7,Ngan Chew Yee7,Daum Chris7,Shapiro Nicole7,Markowitz Victor7,Ivanova Natalia7,Kyrpides Nikos7,Woyke Tanja7,Brown Steven D.145

Affiliation:

1. Graduate School of Genome Science and Technology, University of Tennessee, Knoxville, Tennessee, USA

2. Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel

3. Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Ramat Aviv, Israel

4. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

5. BioEnergy Science Center, Oak Ridge, Tennessee, USA

6. Institute for Environmental Genomics, University of Oklahoma, Norman, Oklahoma, USA

7. DOE Joint Genome Institute, Walnut Creek, California, USA

Abstract

ABSTRACT We and others have shown the utility of long sequence reads to improve genome assembly quality. In this study, we generated PacBio DNA sequence data to improve the assemblies of draft genomes for Clostridium thermocellum AD2, Clostridium thermocellum LQRI, and Pelosinus fermentans R7.

Funder

DOE | SC | Biological and Environmental Research

Publisher

American Society for Microbiology

Subject

Genetics,Molecular Biology

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