Complete Genome Sequence of Escherichia coli BLR(DE3), a recA -Deficient Derivative of E. coli BL21(DE3)-Reference-Cited by-同舟云学术

Complete Genome Sequence of Escherichia coli BLR(DE3), a recA -Deficient Derivative of E. coli BL21(DE3)

Published:2017-06 Issue:22 Volume:5 Page:
ISSN:2169-8287
Container-title:Genome Announcements
language:en
Short-container-title:Genome Announc

Author:

Goffin Philippe¹²,Dehottay Philippe¹

Affiliation:

1. GSK Vaccines, Rixensart, Belgium

2. Laboratoire de Génétique et Physiologie Bactérienne, IBMM, Faculté des Sciences, Université Libre de Bruxelles (ULB), Gosselies, Belgium

Abstract

ABSTRACT Escherichia coli BLR(DE3) is a commercially available recA -deficient derivative of BL21(DE3), one of the most widely used strains for recombinant protein expression. Here, we present the full-genome sequence of BLR(DE3) and highlight additional differences with its parent strain BL21(DE3) which were previously unreported but may affect its physiology.

Publisher

American Society for Microbiology

Subject

Genetics,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/genomeA.00441-17

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