Affiliation:
1. Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York 10032
Abstract
Chloramphenicol-resistant strains of
Staphylococcus aureus
contain an inducible enzyme which inactivates chloramphenicol by acetylation in the presence of acetyl coenzyme A. The products of acetylation are chromatographically indistinguishable from those obtained with chloramphenicol-resistant
Escherichia coli
harboring an R factor. The kinetics of induction of chloramphenicol acetyltransferase are complicated by the inducer's effect on protein biosynthesis and its fate as chloramphenicol 3-acetate, which is not an inducer of the enzyme. The
E. coli
and
S. aureus
enzymes have been compared, with the conclusion that they are identical with respect to molecular weight (approximately 78,000) and
p
H optimum (7.8), but differ with respect to heat stability, substrate affinity, electrophoretic mobility, and immunological reactivity. Antiserum prepared against enzyme from
E. coli
contains precipitating antibody, which inactivates the
E. coli
enzyme, but neither precipitates nor neutralizes the activity of
S. aureus
enzyme.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference20 articles.
1. The order of induction and deinduction of the enzymes of the lactose operon in E. coli;ALPERS D. H.;Proc. Natl. Acad. Sci. U.,1965
2. Variations sous l'influence de I'acriflavine et transduction de la resistance a la kanamycin et au chloramphenicol chez les staphylocoques;CHABBERT Y. A.;Ann. Inst. Pasteur (Suppl.),1964
3. Immunochemistry of enzymes;CINADER B.;Ann. N. Y. Acad. Sci.,1963
4. Disc electrophoresis. II. Method and application to human serum proteins;DAVIS B. J.;Ann. N. Y. Acad. Sci.,1964
5. DUNSMOOR C. L. K. L. PIM AND J. C. SHERRIS. 1964. Observations on the inactivation of chloramphenicol by chloramphenicol-resistant staphylococci. Antimicrobial Agents and Chemotherapy-1963 p. 500-506.
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