Author:
Cooney P H,Whiteman P F,Freese E
Abstract
At some time during sporulation development, cells of Bacillus subtilis develop a commitment to continue sporulation even after addition of or dilution into a fresh nutrient. The extent of commitment was measured by the titer of spores produced at the time at which the original culture sporulated maximally. Since newly formed spores of B. subtilis soon germinate in the replenished medium, the measurement of their titer, especially of heat-resistant spores, gave low values. This problem was avoided by the germination-delaying effect of methyl anthranilate (1 mM) when added together with the fresh nutrients. In a given culture, the titer of committed cells was then independent of the method by which it was measured, i.e., by the phase-bright, octanol-resistant, or heat-resistant spore titer. The time of commitment depended on the type of nutrient added. Commitment occurred earlor casein hydrolysate. The rates at which non-metabolizable amino acid analogues or the 14C from an amino acid mixture were taken up by the cells increased toward the end of growth and later declined. This decline occurred slowly and was only weakly correlated with the commitment time of an analogous amino acid.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference13 articles.
1. Relation between reduced nicotinamide adenine dinucleotide oxidation and amino acid transport in membrane vesicles from Bacillus subtilis;Bisachop A.;J. Bacteriol.,1975
2. Cooney P. H. E. B. Freese and E. Freese. 1975. Commitment ofBacillus megaterium cells to sporulation p. 187-197. In P. Gerhardt R. N. Costilow and H. L. Sadoff (ed.) Spores VI. American Society for Microbiology Washington D.C.
3. Ellar D. J. M. W. Eaton C. Hogarth B. J. Wilkinson J. Deans and J. La Nauze. 1975. Comparative biochemistry and function of forespore and mother-cell compartments during sporulation ofBacillus megaterium cells p. 425-433. In P. Gerhardt R. N. Costilow and H. L. Sadoff (ed.) Spores VI. American Society for Microbiology Washington D.C.
4. Morphogenesis in bacteria: some aspects of spore formation;Foster J. W.;Q. Rev. Biol.,1956
5. Commitment to sporulation and induction of glucose-phosphoenolpyruvate transferase;Freese E.;Biochim. Biophys. Acta,1970
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