Affiliation:
1. Biology Department, The Catholic University of America, Washington, D.C.
Abstract
Beining, Paul
R. (The Catholic University of America, Washington, D.C.)
and E. R. Kennedy
. Characteristics of a strain of
Staphylococcus aureus
grown in vivo and in vitro. J. Bacteriol.
85:
732–741. 1963.—A comparative survey was conducted on the characteristics of a strain of
Staphylococcus aureus
after it had been grown in vitro (VSB) and after it had been collected from the peritoneal exudate of an infected guinea pig (GSB). Both VSB and GSB strains gave the same results when studied in an extensive series of tests, including bound and soluble coagulases, bacteriophage type, antibiotic-sensitivity pattern, the usual fermentation reactions, deoxyribonucleic acid base composition, and qualitative tests for hemolysins, deoxyribonuclease, ribonuclease, staphylokinase, staphyloprotease, lipase, and phosphatase. The in vivo strain differed significantly from the in vitro strain in respiratory rate, agar gel diffusion studies, agglutinability in tube tests, virulence tests in rabbits and mice, growth on tellurite-glycine agar, susceptibility to human γ-globulin in agar, and in the quantitative production of deoxyribonuclease, α-hemolysin, leucocidin, and hyaluronidase.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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