Molecular Characterization of a Variant of Bacillus anthracis -Specific Phage AP50 with Improved Bacteriolytic Activity

Author:

Sozhamannan Shanmuga1,McKinstry Michael1,Lentz Shannon M.1,Jalasvuori Matti2,McAfee Farrell1,Smith Angela1,Dabbs Jason1,Ackermann Hans-W.3,Bamford Jaana K. H.2,Mateczun Alfred1,Read Timothy D.1

Affiliation:

1. Naval Medical Research Center, Silver Spring, Maryland 20910

2. Department of Biological and Environmental Science and Nanoscience Center, P.O. Box 35, 40104-University of Jyväskylä, Jyväskylä, Finland

3. Department of Medical Biology, Faculty of Medicine, Laval University, Quebec, Quebec, Canada G1K 7P4

Abstract

ABSTRACT The genome sequence of a Bacillus anthracis -specific clear plaque mutant phage, AP50c, contains 31 open reading frames spanning 14,398 bp, has two mutations compared to wild-type AP50t, and has a colinear genome architecture highly similar to that of gram-positive Tectiviridae phages. Spontaneous AP50c-resistant B. anthracis mutants exhibit a mucoid colony phenotype.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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