Affiliation:
1. Department of Bacteriology, School of Medicine, Kanazawa University, Kanazawa, Japan
Abstract
A new procedure for isolation of
Clostridium absonum
was devised. Sixtyseven strains of
C. absonum
were isolated from 135 soil samples, but no strain of
C. absonum
could be found from human fecal samples. The lecithinase, hemolysin, and lethal toxin in the culture filtrates of this species exhibited low avidity for
C. perfringens
type A antitoxin. The three activities were inseparable by the present method of purification. A reinvestigation of biochemical properties revealed that incomplete suppression of lecithinase reaction by
C. perfringens
type A antitoxin and no fermentation of raffinose, melibiose, and starch are useful criteria to differentiate
C. absonum
from
C. perfringens
, and that positive, although weak, gelatin liquefaction and fermentation of trehalose are useful to differentiate it from
C. paraperfringens.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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