Role of Escherichia coli DNA Polymerase IV in In Vivo Replication Fidelity-Reference-Cited by-同舟云学术

Role of Escherichia coli DNA Polymerase IV in In Vivo Replication Fidelity

Published:2004-07-15 Issue:14 Volume:186 Page:4802-4807
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Kuban Wojciech¹,Jonczyk Piotr¹,Gawel Damian¹²,Malanowska Karolina¹,Schaaper Roel M.²,Fijalkowska Iwona J.¹

Affiliation:

1. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02 106 Warsaw, Poland

2. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

Abstract

ABSTRACT We have investigated whether DNA polymerase IV (Pol IV; the dinB gene product) contributes to the error rate of chromosomal DNA replication in Escherichia coli . We compared mutation frequencies in mismatch repair-defective strains that were either dinB positive or dinB deficient, using a series of mutational markers, including lac targets in both orientations on the chromosome. Virtually no contribution of Pol IV to the chromosomal mutation rate was observed. On the other hand, a significant effect of dinB was observed for reversion of a lac allele when the lac gene resided on an F′( pro-lac ) episome.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.186.14.4802-4807.2004

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