Affiliation:
1. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo
2. Centro de Biologia Molecular Estrutural, Laboratório Nacional de Luz Síncrotron
3. Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, Brazil
Abstract
ABSTRACT
We have initiated a project to identify protein-protein interactions involved in the pathogenicity of the bacterial plant pathogen
Xanthomonas axonopodis
pv. citri. Using a yeast two-hybrid system based on Gal4 DNA-binding and activation domains, we have focused on identifying interactions involving subunits, regulators, and substrates of the type III secretion system coded by the
hrp
(for hypersensitive response and pathogenicity),
hrc
(for
hrp
conserved), and
hpa
(for
hrp
associated) genes. We have identified several previously uncharacterized interactions involving (i) HrpG, a two-component system response regulator responsible for the expression of
X. axonopodis
pv. citri
hrp
operons, and XAC0095, a previously uncharacterized protein encountered only in
Xanthomonas
spp.; (ii) HpaA, a protein secreted by the type III secretion system, HpaB, and the C-terminal domain of HrcV; (iii) HrpB1, HrpD6, and HrpW; and (iv) HrpB2 and HrcU. Homotropic interactions were also identified for the ATPase HrcN. These newly identified protein-protein interactions increase our understanding of the functional integration of phytopathogen-specific type III secretion system components and suggest new hypotheses regarding the molecular mechanisms underlying
Xanthomonas
pathogenicity.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference69 articles.
1. Aldon, D., B. Brito, C. Boucher, and S. Genin. 2000. A bacterial sensor of plant cell contact controls the transcriptional induction of Ralstonia solanacearum pathogenicity genes. EMBO J.19:2304-2314.
2. Alfano, J. R., and A. Collmer. 2001. Mechanisms of bacterial pathogenesis in plants: familiar foes in a foreign kingdom, p. 179-226. In E. A. Groisman (ed.), Principles of bacterial pathogenesis. Academic Press, Inc., New York, N.Y.
3. Bacterial secrets of secretion: EuroConference on the biology of type IV secretion processes
4. Bartel, P. L., J. A. Roecklein, D. SenGupta, and S. Fields. 1996. A protein linkage map of Escherichia coli bacteriophage T7. Nat. Genet.12:72-77.
5. Bejar, S., K. Cam, and J. P. Bouche. 1986. Control of cell division in Escherichia coli: DNA sequence of dicA and of a second gene complementing mutation dicA1, dicC. Nucleic Acids Res.14:6821-6833.
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