Coassembly of SecYEG and SecA Fully Restores the Properties of the Native Translocon-Reference-Cited by-同舟云学术

Coassembly of SecYEG and SecA Fully Restores the Properties of the Native Translocon

Published:2019-01 Issue:1 Volume:201 Page:
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Bariya Priya¹,Randall Linda L.¹

Affiliation:

1. Department of Biochemistry, University of Missouri, Columbia, Missouri, USA

Abstract

This work presents a comprehensive quantification of the parameters of transport by the Sec general secretory system in the three in vitro systems. The standard reconstitution used by most investigators can be enhanced to yield six times as many active translocons simply by adding SecA to SecYEG during reconstitution. This robust system faithfully reflects the properties of translocation in native membrane vesicles. We have expanded the number of precursors studied to nine. This has allowed us to conclude that the rate constant for translocation varies with precursor species.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00493-18

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