Development and Validation of a Competitive ELISA Based on Bovine Monoclonal Antibodies for the Detection of Neutralizing Antibodies against Foot-and-Mouth Disease Virus Serotype A-Reference-Cited by-同舟云学术

Development and Validation of a Competitive ELISA Based on Bovine Monoclonal Antibodies for the Detection of Neutralizing Antibodies against Foot-and-Mouth Disease Virus Serotype A

Published:2022-04-20 Issue:4 Volume:60 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cao Yimei¹,Li Kun¹,Xing Xiangchuan¹²,Zhu Guoqiang¹,Fu Yuanfang¹,Bao Huifang¹,Bai Xingwen¹,Sun Pu¹,Li Pinghua¹,Zhang Jing¹,Ma Xueqing¹,Wang Jian¹,Zhao Zhixun¹,Li Dong¹,Liu Zaixin¹,Lu Zengjun¹³^ORCID

Affiliation:

1. State Key Laboratory of Veterinary Etiological Biology, OIE/National Foot-and-Mouth Disease Reference Laboratory of China, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China

2. Tianjin Weite Biological Medicine Co., Ltd., Tianjin, China

3. College of Veterinary Medicine, Lanzhou University, Lanzhou, China

Abstract

The level of neutralizing antibodies in vaccinated animals is directly related to their level of protection against a virus challenge. The virus neutralization test (VNT) is a “gold standard” method for detecting neutralizing antibodies against foot-and-mouth disease virus (FMDV). However, VNT requires high-containment facilities that can handle live viruses and is not suitable for large-scale serological surveillance. In this study, a bovine broadly neutralizing monoclonal antibody (W145) against FMDV serotype A was successfully produced using fluorescence-based single-B-cell antibody technology.

Funder

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.02142-21

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