Function of Ech Hydrogenase in Ferredoxin-Dependent, Membrane-Bound Electron Transport in Methanosarcina mazei-Reference-Cited by-同舟云学术

Function of Ech Hydrogenase in Ferredoxin-Dependent, Membrane-Bound Electron Transport in Methanosarcina mazei

Published:2010-02 Issue:3 Volume:192 Page:674-678
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Welte Cornelia¹,Kallnik Verena¹,Grapp Marcel²,Bender Gunes³,Ragsdale Steve³,Deppenmeier Uwe¹

Affiliation:

1. Institute of Microbiology and Biotechnology, University of Bonn, Meckenheimer Allee 168, 53115 Bonn, Germany

2. Zentrum für Kinderheilkunde und Jugendmedizin, Universität Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany

3. Department of Biological Chemistry, University of Michigan Medical School, 1150 W. Medical Center Dr., Ann Arbor, Michigan 48109-0606

Abstract

ABSTRACT Reduced ferredoxin is an intermediate in the methylotrophic and aceticlastic pathway of methanogenesis and donates electrons to membrane-integral proteins, which transfer electrons to the heterodisulfide reductase. A ferredoxin interaction has been observed previously for the Ech hydrogenase. Here we present a detailed analysis of a Methanosarcina mazei Δ ech mutant which shows decreased ferredoxin-dependent membrane-bound electron transport activity, a lower growth rate, and faster substrate consumption. Evidence is presented that a second protein whose identity is unknown oxidizes reduced ferredoxin, indicating an involvement in methanogenesis from methylated C 1 compounds.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.01307-09

Reference28 articles.

1. Isolation and Characterization of Methanophenazine and Function of Phenazines in Membrane-Bound Electron Transport of Methanosarcina mazei Gö1

2. Bäumer, S., T. Ide, C. Jacobi, A. Johann, G. Gottschalk, and U. Deppenmeier. 2000. The F420H2 dehydrogenase from Methanosarcina mazei is a redox-driven proton pump closely related to NADH dehydrogenases. J. Biol. Chem. 275 : 17968-17973.

3. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

4. Brodersen, J., G. Gottschalk, and U. Deppenmeier. 1999. Membrane-bound F420H2-dependent heterodisulfide reduction in Methanococcus voltae. Arch. Microbiol. 171 : 115-121.

5. Deppenmeier, U., A. Johann, T. Hartsch, R. Merkl, R. A. Schmitz, R. Martinez-Arias, A. Henne, A. Wiezer, S. Bäumer, C. Jacobi, H. Brüggemann, T. Lienard, A. Christmann, M. Bömeke, S. Steckel, A. Bhattacharyya, A. Lykidis, R. Overbeek, H. P. Klenk, R. P. Gunsalus, H. J. Fritz, and G. Gottschalk. 2002. The genome of Methanosarcina mazei: evidence for lateral gene transfer between bacteria and archaea. J. Mol. Microbiol. Biotechnol. 4 : 453-461.

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