Ethylene Glycol Metabolism by Pseudomonas putida

Author:

Mückschel Björn1,Simon Oliver2,Klebensberger Janosch1,Graf Nadja3,Rosche Bettina4,Altenbuchner Josef3,Pfannstiel Jens2,Huber Armin2,Hauer Bernhard1

Affiliation:

1. Institute of Technical Biochemistry, University of Stuttgart, Stuttgart, Germany

2. Department of Biosensorics and Proteomics Core Facility of Life Science Center, University of Hohenheim, Stuttgart, Germany

3. Institute of Industrial Genetics, University of Stuttgart, Stuttgart, Germany

4. School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, Australia

Abstract

ABSTRACT In this study, we investigated the metabolism of ethylene glycol in the Pseudomonas putida strains KT2440 and JM37 by employing growth and bioconversion experiments, directed mutagenesis, and proteome analysis. We found that strain JM37 grew rapidly with ethylene glycol as a sole source of carbon and energy, while strain KT2440 did not grow within 2 days of incubation under the same conditions. However, bioconversion experiments revealed metabolism of ethylene glycol by both strains, with the temporal accumulation of glycolic acid and glyoxylic acid for strain KT2440. This accumulation was further increased by targeted mutagenesis. The key enzymes and specific differences between the two strains were identified by comparative proteomics. In P. putida JM37, tartronate semialdehyde synthase (Gcl), malate synthase (GlcB), and isocitrate lyase (AceA) were found to be induced in the presence of ethylene glycol or glyoxylic acid. Under the same conditions, strain KT2440 showed induction of AceA only. Despite this difference, the two strains were found to use similar periplasmic dehydrogenases for the initial oxidation step of ethylene glycol, namely, the two redundant pyrroloquinoline quinone (PQQ)-dependent enzymes PedE and PedH. From these results we constructed a new pathway for the metabolism of ethylene glycol in P. putida . Furthermore, we conclude that Pseudomonas putida might serve as a useful platform from which to establish a whole-cell biocatalyst for the production of glyoxylic acid from ethylene glycol.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference40 articles.

1. Reaction of glycolaldehyde with proteins: latent crosslinking potential of alpha-hydroxyaldehydes;Acharya AS;Proc. Natl. Acad. Sci. U. S. A.,1983

2. AdachiO AnoY ToyamaH MatsushitaK. 2007. Biooxidation with PQQ- and FAD-dependent dehydrogenases, p 1–35. In SchmidRD UrlacherVB (ed), Modern biooxidation: enzymes, reactions, and applications. Wiley-VCH Verlag GmbH & Co KGaA, Weinheim, Germany.

3. Genetic analyses and molecular characterization of the pathways involved in the conversion of 2-phenylethylamine and 2-phenylethanol into phenylacetic acid in Pseudomonas putida U;Arias S;Environ. Microbiol.,2008

4. The metabolism of glyoxylate by cell-free extracts of Pseudomonas sp;Bailey E;Biochem. J.,1966

5. Microbial growth on oxalate by a route not involving glyoxylate carboligase;Blackmore MA;Biochem. J.,1970

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