Selection and Characterization of a Multivalent Salmonella Phage and Its Production in a Nonpathogenic Escherichia coli Strain

Author:

Santos S. B.1,Fernandes E.1,Carvalho C. M.1,Sillankorva S.1,Krylov V. N.2,Pleteneva E. A.2,Shaburova O. V.2,Nicolau A.1,Ferreira E. C.1,Azeredo J.1

Affiliation:

1. IBB—Institute for Biotechnology and Bioengineering, Center of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4700-057 Braga, Portugal

2. Institute of Genetics and Selection of Industrial Microorganisms, Moscow 117454, Russia

Abstract

ABSTRACT We report the selection and amplification of the broad-host-range Salmonella phage phi PVP-SE1 in an alternative nonpathogenic host. The lytic spectrum and the phage DNA restriction profile were not modified upon replication in Escherichia coli Bl21, suggesting the possibility of producing this phage in a nonpathogenic host, contributing to the safety and easier approval of a product based on this Salmonella biocontrol agent.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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