False-Negative Results Obtained with the Gen-Probe Amplified Mycobacterium tuberculosis Direct Test Caused by Unrecognized Inhibition of the Amplification Reaction-Reference-Cited by-同舟云学术

False-Negative Results Obtained with the Gen-Probe Amplified Mycobacterium tuberculosis Direct Test Caused by Unrecognized Inhibition of the Amplification Reaction

Published:2009-09 Issue:9 Volume:47 Page:2995-2997
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kim Ji Yeon¹,Ferraro Mary Jane¹²,Branda John A.¹

Affiliation:

1. Departments of Pathology

2. Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Abstract

ABSTRACT The Gen-Probe Amplified Mycobacterium tuberculosis direct test is widely used in the diagnosis of tuberculosis. Specimens may contain amplification inhibitors, potentially leading to false-negative results if unrecognized. We report a failure to detect inhibition, despite adherence to the inhibition testing guidelines provided on the label, and recommend changes.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00966-09

Reference19 articles.

1. Alcalá, L., M. J. Ruiz-Serrano, S. Hernangómez, M. Marín, D. García de Viedma, R. San Juan, and E. Bouza. 2001. Evaluation of the upgraded amplified Mycobacterium tuberculosis direct test (Gen-Probe) for direct detection of Mycobacterium tuberculosis in respiratory and non-respiratory specimens. Diagn. Microbiol. Infect. Dis.41:51-56.

2. Centers for Disease Control and Prevention. 1996. Nucleic acid amplification tests for tuberculosis. MMWR Morb. Mortal. Wkly. Rep.45:950-952.

3. Reported tuberculosis in the United States 1993. 1994

4. Centers for Disease Control and Prevention. 2000. Update: nucleic acid amplification tests for tuberculosis. MMWR Morb. Mortal. Wkly. Rep.49:593-594.

5. Centers for Disease Control and Prevention. 2009. Updated guidelines for the use of nucleic acid amplification tests in the diagnosis of tuberculosis. MMWR Morb. Mortal. Wkly. Rep.58:7-10.

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